In the process of carbon fixation, RuBP attaches to a CO2 molecule to produce a 6-carbon compound, which is then split in two. After phosphorylation and reduction, what more needs to happen in the Calvin cycle?

A. Regeneration of RuBP
B. Addition of a pair of electrons from NADPH
C. Inactivation of RuBP carboxylase enzyme
D. Regeneration of ATP from ADP
E. A gain of NADPH

Phosphorylation and reduction in the Calvin cycle, the key process that needs to occur is the regeneration of RuBP, which prepares the system for more CO2 to be fixed, thus continuing the cycle.

Explanation:

In the Calvin cycle, after the process of carbon fixation where RuBP attaches CO2 to produce a 6 carbon molecule that gets split, and subsequent phosphorylation and reduction, the cycle goes into its final stage: regeneration. Here, RuBP, which initially kicked off the cycle, is regenerated using ATP. This ensures the continuance of the Calvin cycle as it prepares for more CO2 to be fixed. In sum, it's the regeneration of RuBP that needs to happen after phosphorylation and reduction within the Calvin cycle.

Learn more about Calvin Cycle here:

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